Therefore, the virus sample preservation solution added with guanidine hydrochloride or guanidine isothiocyanate is the inactivated virus sample preservation solution. The main purpose of the Tris, guanidine hydrochloride or guanidine isothiocyanate, EDTA, etc., is to lyse nucleic acid and release it. The nucleic acid is extracted, and the subsequent real-time fluorescent RT-PCR is used for nucleic acid detection to determine whether the sample contains virus characteristic nucleic acid, that is, whether it is infected with the virus.
Nucleic acid is a biological macromolecular compound composed of many nucleotides, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and is one of the basic substances of life. The virus has a single structure and contains only one nucleic acid and protein, so the detection of the characteristic nucleic acid can detect the virus. Viruses are parasitic organisms and cannot survive in vitro after sampling. If they cannot be detected in time, they need to be placed in the virus sample preservation solution. In order to protect the safety of the virus detection environment, it is necessary to add guanidine hydrochloride or guanidine isothiocyanate to inactivate the virus and release nucleic acids that can be detected.
Guanidine is a nitrogen-containing organic compound, also known as "iminourea", "iminocarbazide", and "carbamidine". Guanidine hydrochloride or guanidine isothiocyanate are strong inhibitors of nucleases, facilitating the extraction of intact RNA from RNASE-rich tissues. Guanidine hydrochloride or guanidine isothiocyanate can not only quickly destroy cell membranes, but also denature proteins, so that proteins can be denatured and precipitated, so that nucleic acids can get rid of the entanglement of proteins. The inactivated virus preservation solution containing guanidine hydrochloride or guanidine isothiocyanate can fully and effectively lyse cells, so that the nucleic acid in the cells can be fully released, and a higher concentration of nucleic acid can be obtained, which is conducive to improving the quality of nucleic acid and ensuring the subsequent operation. accuracy.