Guanidine hydrochloride is a powerful chaotropic agent. It not only promotes the solubility of hydrophobic molecules, but also can be used for protein denaturation and subsequent denaturation. In a high-concentration guanidine hydrochloride solution, the ordered "secondary structure" of all proteins (maintained by intermolecular non-covalent interactions, such as hydrogen bonds and hydrophobic bonds) is destroyed, peptide chains are extended, and tertiary structure is destroyed . Change. Guanidine hydrochloride can dissolve insoluble or denatured proteins, such as inclusion bodies, and recover periplasmic proteins. It can also be used for protein development or the first step of enzymatic folding, which may require urea and DTT. Guanidine hydrochloride is also commonly used in RNA purification to dissociate nuclear proteins and inhibit RNase activity. Guanidine hydrochloride is mainly used as an intermediate in medicine, pesticide, dye and other organic synthesis. Guanidine hydrochloride is an important raw material for the production of sulfadiazine, sulfamethazine, sulfamethazine and folic acid. It can also be used as an antistatic agent for synthetic fibers. In addition, guanidine hydrochloride can also be used as a detection reagent for the detection of precious metals such as gold, iridium, os, platinum, and ruthenium.
